• 陆祖宏
    博士、教授、博士生导师
    生物电子学国家重点实验室主任
    zhlu@seu.edu.cn
  •     实验室主页

      

    研究方向

      

    • 生物芯片和DNA存储

    • 新一代DNA测序技术

    • 生物信息学和健康信息技术



    教育经历


    198812月东南大学生物科学与医学工程系, 博士学位

    19846月东南大学电子工程系, 硕士学位

    19822月东南大学电子工程系,工学学士


    研究成果

      

    首批获教育部跨世纪优秀人才基金,国家自然科学基金优秀中青年基金,国家自然科学基金杰出人才基金,首批教育部“**学者奖励计划”特聘教授。2002年国家自然科学基金创新研究群体项目负责人。曾获教育部科技进步二等奖3项、三等奖3项。指导了三篇全国优秀博士论文。中国电子学会生物医学电子学分会主任委员;国务院学位委员会第六届学科评议组成员,科技部863“十二五生物医药领域前沿生物技术主题专家组成员;国际无线电科学联盟中国委员会委员等。国际学术杂志“IEEE Transaction on Nanobioscience”、“IET nanobiotechnology”、《科学通报》、《仪器仪表学报》、《遗传学报》等学术刊物的编委等。多年来在新一代高通量DNA测序技术、新型生物芯片及其应用、以及生物信息分析等相关领域中取得了多项研究成果,在国际学术刊物上发表论文100多篇,获国家发明专利10多项,其中获美国发明专利1项。申请了国家发明专利20多项。

    近十年的主要研究成果包括:提出并发展了硫代碱基切割合成测序、组合标记连接测序以及多重解码合成测序等新型的二代高通量低成本DNA测序技术,设计和研制了新一代高通量DNA测序的实验样机和测序芯片,发展了单分子多拷贝DNA测序文库的制备方法,开展了miRNAChIP-SeqCNVs,微生物16s RNAmRNA,模式生物基因组等测序,具备测序成本低、速度快、精确度高等优势。发展了甲基化检测芯片、双链DNA芯片、PCR产物微阵列芯片、Eppendorf管帽芯片( microarray-in-a-tube) 、荧光定量PCR微阵列芯片、免疫捕获细胞微阵列芯片等多种新型生物芯片技术,并应用于大量临床样本的检测。

      

    主要获奖及荣誉

      

    2001年宝钢教学特等奖

    2004年江苏省青年科学家奖

    2007江苏省333高层次人才培养工程中青年首席科学家

    2007获全国模范教师称号

      

    发明专利

      

    多次压印定点合成法制备化合物微阵列芯片的方法,专利号:98111220X,国际专利号:PCT/CN99/00013,美国专利号:US09/647525.

    细胞微阵列芯片及其制备方法,国家专利号:00112363.7

    基因扩增,杂交检测一体化的反应管帽,国家专利号01217502.1

    DNA甲基化检测方法,国家专利号02148401.5

    用于细胞或微生物检测的微阵列芯片及其制备方法,国家专利号02138639.0

    一种非标记检测DNA结合蛋白的芯片及其制备、应用方法,国家专利号:03112917.X

    分子印章测序装置和测序方法, 国家专利号:200610096538.2

    一种反向杂交偶联延伸DNA测序方法,国家专利号:200710131536.7

    基于碱基修饰保护往复延伸的DNA测序方法,国家专利号:200710131550.7

    待测核酸序列的编码和解码方法,国家专利号200710135581.X

    利用硫代寡核苷酸探针的DNA测序方法,国家专利号:200810019782.8

    带背景验证的信号组合编码DNA连接测序方法,国家专利号:200910026890.2

    可二次验证碱基信息的DNA测序方法,国家专利号:200910033400.1

    一种提高核酸内切酶V切割位置特异性的方法,国家专利号:200910145186.9

    一种用于核酸测序的微流体基因芯片,国家专利号201010288225.3

    可进行单分子核酸扩增的颗粒、其制备方法及应用,国家专利号:201110291304.4

    两核苷酸同时合成DNA测序方法及其应用,国家专利号:201110321795.2

    一种微流控芯片及其制作方法,国家专利号:201310571537.9

    高通量STR序列核心重复数检测方法,国家专利号:201410410187.2

    一种两轮信号耦合编码的DNA连接测序方法,国家专利号:201410599337.9

    一种用于细胞捕获的微流控芯片,国家专利号:201410605526.2

    一种用于微控芯片的单向球阀及其制备方法,国家专利号:201510385896.4

      

    主要发表论文

      

    (1) H.Wang, J.Li, H.Liu, Q.Liu, Q.Mei, Y.Wang, J.Zhu, N.He and Z.LuLabel-free hybridization detection of a single nucleotide mismatch by immobilization of molecular beacons on an agarose filmNucleic Acid Research.2002,30:e61×

    (2) P.Xiao, N.He,andZ.Lu, In situ synthesis of oligonucleotide arrays by using soft lithography. Nanotechnology.2002,13:756-762

    (3) J.Ma, T.Zhou, W.Gu, X.Sunand Z.LuCluster analysis of the codon use frequency of MHC genes from different species,Biosystems.2002, 65:199-207

    (4) P.Hou, M.Ji, C.Ge, J.Shen, S.Li, N.He and Z.Lu, Detection of methylation of human p16Ink4a gene 5’-CpG islands by electrochemical method coupled with linker-PCR, Nucleic Acids Research. 2003,31:e92

    (5) C. Zhang, H. Liu, Z. Tang, N. He, and Z. Lu, Cell detection based on protein array using modified glass slides, Electrophoresis. 2003, 24:3279-3283

    (6) T. Li, J. Wang, Y. Bai, X. Sun, and Z. Lu, A novel method for screening species-specific gDNA probes for species identification, Nucleic Acids Research. 2004, 32:e42

    (7) W. Gu, T. Zhou, J. Ma, Xiao Sun, and Z. Lu. Analysis of synonymous codon usage in SARS coronavirus and other viruses in the nidovirales, Virus Research. 2004, 101: 155–161

    (8)Q. Liu, Q. Zhou, Y. Bai, Q. Ge, and Z. Lu, Detection and analysis system for hybridization images of Lab-in-a-Tube microarray Chinese Science Bulletin. 2005, 50: 2896-2900

    (9)H. Liu, H. Wang, Z. Shi, H. Wang, C. Yang, S. Silke, W. Tan and Z. Lu, TaqMan probe array for quantitative detection of DNA targets, Nucleic Acids Research. 2006, 34: e4

    (10)P.Hou, M. Ji, B. Yang, Z. Chen, J. Qiu, X. Shi, and Z. Lu, Quantitative Analysis of Promoter Hypermethylation in Multiple Genes in Osteosarcoma, Cancer. 2006, 106:1602-1609

    (11)T. Zhou, X. Sun, Z. Lu, “Synonymous codon usage in environmental chlamydia UWE25 reflects an evolutional divergence from pathogenic chlamydiae ”GENE. 2006, 368:117-125

    (12)P. Jiang, H. Wu, W. Wang, W. Ma, X. Sun, Z. Lu, MiPred: classification of real and pseudo microRNA precursors using random forest prediction model with combined features, Nucleic Acids Research. 2007, 35: W339-W344

    (13)P. Jiang, H. Wu, J. Wei, F. Sang, X. Sun, and Z. Lu, RF-DYMHC: detecting the yeast meiotic recombination hotspots and coldspots by random forest model using gapped dinucleotide composition features, Nucleic Acids Research. 2007, 35: W47-W51

    (14)Q. Liu, Y. Bai, Q. Ge, S. Zhou, T. Wen, and Z. LuMicroarray-in-a-tube for Detection of Multiple Viruses, Clinical Chemistry. 2007, 53:188-194

    (15)Y. Wan, Y. Wang, P. Xiao, Z. Lu, Bisulfite modification of immobilized DNAs for methylation detection, Biosensors and Bioelectronics. 2007, 22:2415-2421

    (16)P.Hou, Z. Chen, M. Ji, N. He, Z. Lu, Real-time PCR Assay for Ultrasensitive Quantification of DNA-Binding Proteins, Clinical Chemistry, 2007 53, 581–586

    (17)D. Zhang, Y. Wang, Y. Bai, Q. Ge, Y. Qiao, J. Luo, C. Jia, and Z. LuA novel method to quantify local CpG methylation density by regional methylation elongation assay on microarrayBMC Genomics. 2008, 9:59

    (18)Y. Wang, D. Zhang, W. Zheng, J. Luo, Y. Bai, and Z. Lu, Multiple gene methylation of nonsmall cell lung cancers evaluated with 3-dimensional microarrayCancer. 2008, 112:1325-1336

    (19)J. Tang, Y. Li, Z. Pan, Y. Guo, J. Ma, S. Ning, P. Xiao, and Z. Lu, Single nucleotide variations detection by ligation of universal probes on 3-D polyacrylamide gel DNA microarray, Human mutation. 2009, 30:1460-1468

    (20)Y. Yang, W. Wang, Y. Li, J. Tu, Y. Bai, P. Xiao, D. Zhang, and Z. Lu, Identification of methylated regions with peak search based on Poisson model from massively parallel methylated DNA immunoprecipitation -sequencing data, Electrophoresis.2010,31:35373544

    (21)C. Tang,X. Shi, W. Wang, D. Zhou, J. Tu, X. Xie, Q. Ge, P. Xiao, X. Sun, and Z. Lu, Global analysis of in vivo EGR1-binding sites in erythroleukemia cell using chromatin immunoprecipitation and massively parallel sequencingElectrophoresis. 2010, 31:2936–2943

    (22)W. Wang, X. Shi, and Z. LuSimulation of ChIP-Seq based on extra-sonication of IPed DNA fragments, Chinese Science Bulletin. 2010,55:2380-2389

    (23)L.Guo, T. Liang, Z. Lu, A comprehensive study of multiple mapping and feature selection for correction strategy in the analysis of small RNAs from SOLiD sequencing. BioSystems, 2011,104: 87-93

    (24)J.Tu, Q. Ge, S. Wang, L. Wang, B. Sun, Q. Yang, Y. Bai, and Z. Lu, Pair-barcode high-throughput sequencing for large-scale multiplexed sample analysis. BMC Genomics. 2012, 13(1):43

    (25)X.Ma, W. Xu, C. Chen, Z. Lu, andJ. Li,A microfabrication-free nanoliter droplet array for nucleic acid detection combined with isothermal amplification. Analyst, 2015, 140(13) 4370-4373.

    (26)F.Liang, A. Ju, Y. Qiao, J. Guo, H. Feng, J. Li, N. Lu, J. Tu, and Z. Lu, A simple approach for an optically transparent nanochannel device prototype. Lab on a Chip, 2016, 16(6):984-991

    (27)J.Li, N. Lu, X. Shi, Y. Qiao, L. Chen, M. Duan, Y. Hou, Q. Ge, Y. Tao, J.Tu, and Z. Lu, 1D-reactor Decentralized MDA for Uniform and Accurate Whole Genome Amplification. Analytical Chemistry, 2017, 89(19), 10147-10152.